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1.
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Molecular Epidemiology Of Subclinical Tuberculosis In Peri-Urban Human Population Of Lahore.

by Sadeem Shahzad | Dr. Muhammad Yasir Zahoor | Dr. Muhammad | Prof. Dr. Tahir Yaqub.

Material type: book Book; Format: print ; Literary form: drama Publisher: 2013Dissertation note: Tuberculosis (TB) is known to be a major health problem worldwide causing disease among millions of people every year. Major cause of tuberculosis in human is the infection with M.tuberculosiswhich usually causes pulmonary or lungs TB but an unknown number of patients are also infected with M.bovis which causes tuberculosis in humans as zoonotic agent along with its major hosts like cattle and deer. In developing countries where raw milk is used without pasteurisation there is a heavy risk of tuberculosis infection with M.bovis. TB infection with M.bovis mainly appears as extra pulmonary tuberculosis with and without specific symptoms of the disease.Diagnosis of subclinical asymptomatic tuberculosis and that of extra pulmonary tuberculosis is a difficult task and most of the time disease remains undiagnosed or misdiagnosed due to the unavailability of specific and sensitive diagnostic tool to diagnose the disease at early stage. Moreover prevalence of M.bovisinfection is not properly known. This study was designed to measure the diagnostic value of Interferon gamma release assay (IGRA) for early and reliable diagnosis of subclinical extra pulmonary TB along with the molecular epidemiology of subclinical extra pulmonary TB to check the prevalence of M.bovisinfection. IGRA is a latest blood test with high specificity and sensitivity based on the principle of Interferon gamma released by effector T-Cell when exposed to M.tuberculosis antigens like ESAT-6 and CFP-10 in controlled in-vitro conditions. Eighty patients were selected for the study on the bases of the history of having day to day cattle contact along with feelings of sickness. Biopsy tissue samples of all the patients which were positive with IGRA were requested, however 24 out of 27 positive samples were collected and were first examined histologically. Twenty seven samples out of eighty were found positive with IGRA while 22 out of 24 samples were confirmed by histological examination as infected with MTB. Both IGRA and histological examination are unable todifferentiate between the specie specific infection with M.tuberculosis orM.bovis for which differential amplification of specific fragments of bothof the species was done by running a multiplex PCR using M.tuberculosis specific 185 bp pncA product and M.bovis specific 500 bp segment. Genomic DNA was extracted from previously formalin fixed paraffin embedded (FFPE) tissues which requires pretreatment for deparaffinization. Xylene was used as deparaffinization agent. All of the twenty two samples positive with IGRA and histological study were found positive for M.tuberculosis infection and none of the sample was found positive for M.bovis infection. Results showeda close correlation among all three techniques with their specific benefits and limitations. Study concluded that T.Spot TB (IGRA) is a potentially reliable test for the diagnosis of subclinical, extrapulmonary TB.Formalin Fixed Paraffin Embedded (FFPE) tissues may be used for TB diagnosis and other DNA based researches. Duplex PCR is a reliable technique for differential diagnosis of infection with different species of MTB complex, though none of the sample was found positive for M.boviswhich is may be due to small sample size of the study and it may further be studied in future researches. The research findings will help the clinicians to depend on IGRA testing for timely and reliable diagnosis of extrapulmonary subclinical tuberculosis and potential use of FFPE tissue samples as appropriate specimen for molecular based diagnosis of TB. Further studies are however, required to check the prevalence of M.bovis infection byincreasing sample size. Availability: Items available for loan: UVAS Library [Call number: 1621,T] (1).

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2.
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The Study Of Retinoblastoma (Rb) Gene Mutations Involved In Different Types Of Cancers In Dogs And Cats.

by Siddra Pervaiz | Dr. Muhammad Wasim | Dr. Muhammad | Dr. Muhammad Yasir Zahoor.

Material type: book Book; Format: print ; Literary form: not fiction Publisher: 2013Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1632,T] (1).

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3.
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Molecular Characterization Of Cldn 14 Gene Encoding A Cell Tight Junction Protein In Mouse

by Ihsan Ullah | Dr. Muhammad Yasir zahoor | Dr. Wasim Shehzad | Ms. Shagufta.

Material type: book Book; Format: print Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1906,T] (1).

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4.
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Molecular Diversity Of Fumaryl Acetoacetate Hydrolase Gene In Mammalian Species

by Sadaqat ijaz | Dr. Muhammad yasir zahoor | DR. Muhammad Imran.

Material type: book Book; Format: print Publisher: 2014Dissertation note: The present study has been planned to study the pathogenicity of FAdv-4 by inoculation of different age groups of broiler birds through different parenteral routes and oronasal routes. The liver homogenate suspension prepared from infected liver samples and cell culture propagated infectious agents were used to infect the susceptible broiler birds via parenteral routes and through oronasal routes. For this purpose two experiments were designed as Experiment I and II. In Experiment I the 25-day-old broiler birds were inoculated with different dilutions of liver homogenate and cell culture propagated HPS virus through intramuscular (i/m) and oral routes. Similarly in Experiment II the one-day-old, 1-week-old, 2-week-old, 3-week-old and 4-week-old broiler chickens were inoculated with the original dilution (100) of same liver homogenate and cell culture propagated HPS virus through S/C and oral route. The birds were kept under observation for recording morbidity and mortality. In Experiment I the liver homogenate caused 64% mortality in broiler birds of the Group A through intramuscular route, while 33.33% mortality in broiler birds of Group B through oral route. The cell culture propagated HPS virus caused 60% and 13.33% mortality in broiler birds of Group C and D through intramuscular and oral routes, respectively. In Experiment II none of the day-old-chick died from Group A inoculated with liver homogenate and cell culture propagated HPS virus through s/c and oral route. The liver homogenate and cell culture propagated HPS virus caused high mortality in different age groups of broiler birds through s/c route than oral route. The blood samples were collected from the broiler birds before and after infection and various hematological parameters such as Hemoglobin and packed cell volumes were studied. The values of hemoglobin and packed cell volume showed highly significant (P<0.05) reduction indicating anaemia. The values of hemoglobin and packed cell volume of the broiler birds inoculated with infectious liver homogenate showed highly significant reduction than the birds inoculated with cell culture propagated HPS virus. The results indicated that the liver homogenate is more pathogenic than cell culture propagated HPS virus. There changes may be due to adoptability of the original FAdVs after continued passages in the culture of chicken embryo liver cells. Availability: Items available for loan: UVAS Library [Call number: 0946,T] (1).

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5.
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Molecular Phylogeny And Diversity Analysis Of Gazella Bennettii (Chinkara) Deer Of Pakistan Using D- Loop Region Of Mitochondrial DNA

by Maleekah zaheer | Dr. Muhammad yasir zahoor | DR. Ali raza awan | Miss. Faiza.

Material type: book Book; Format: print ; Literary form: not fiction Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1985,T] (1).

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6.
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Analysis Of Epidermal Growth Factor Receptor Gene Polymorphism Implicated In Tumors Including Oralsquamous Cell

by Noveen nawaz | Dr. Muhammad Wasim | Dr. Muhammad | Dr. Muhammad Yasir zahoor.

Material type: book Book; Format: print Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1998,T] (1).

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7.
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Molecular Analysis Of Mitochondrial Hypervariable Region In Three Consecutive Generations Of Buffalo

by Zara zaheer | Dr. Muhammad Yasir zahoor | Dr. MUhammad Imran | MR. Tariq.

Material type: book Book; Format: print ; Literary form: not fiction Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 2003,T] (1).

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8.
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In- Silico Functional Prediction Of Prion Protein Polymorphisms In Bovine Spongiform Encephalopathy

by Sana jafar | DR. Nuhammad imran | Dr. Muhammad yasir zahoor | Ms. Faiza.

Material type: book Book; Format: print Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 2011,T] (1).

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9.
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In Silico Functional Prediction Of Prion Protein Polymorphisms In Chronic Qasting Disease

by Iqra khizar | DR. Muhammad imran | Dr. Muhammad | Dr. Muhammad yasir zahoor.

Material type: book Book; Format: print ; Literary form: not fiction Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 2024,T] (1).

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